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Oyster mushroom production

Commercial production of oyster mushroom including season, varieties, spawn production, mushroom shed making, harvesting etc are covered

Season and Varieties

  • mushroom cultivated throughout the year
  • Cultivation is indoor and it requires mushroom house
  • White oyster (Co-1) and Grey Oyster (M-2) are suitable for Tamil Nadu


Mushroom House

  • A thatched Shed of 16 sq.m. is required. Divide the shed into spawn running and cropping rooms
  • Spawn running room: maintain 25-300C, provide ventilation, no light is required
  • Cropping room: Maintain 23-250C, RH above 75-80% with moderate light and aeration.

(Digital Thermometers and Humidity meters are available in the market)

Spawn (Mushroom Seeding)

  • Suitable substrate: Sorghum, Maize or, Wheat grains
  • Preparation of spawn: Half cooked grains, air dried, mixed with calcium carbonate powder at 2% level, fill the grains in empty glucose drip bottles, plug with cotton and sterilize in cooker for 2 hours.
  • Put the pure culture of the fungus (Procured from agriculture departments/agrl. Universities) and incubate at room temperature for 15 days. Use 15-18 days old spawn for spawning.

Preparation of Mushroom bed

  • Suitable substrate: Paddy/wheat straw, sugarcane baggasse, hulled maize cobs
  • Cooking of substrate: Cut into 5cm bits, soak in potable water for 5 hrs, boil water for one hour, drain the water, air dry to 65% moisture (no water drips when squeezed between hands)
  • Preparation of bags:
    1. Use 60 ×30 cm polythene bags (both side open).
    2. Keep the beds moist by periodical spraying with water.
    3. Tie one end of bag, put two holes of 1 cm dia in the middle.
    4. Put handful of cooked straw in the bag to a height of 5 cm; sprinkle about 25 g of spawn.
    5. Layer the straw to 25 cm height. Repeat the process to get four layers of spawn and 5 layers of straw.
    6. Tie the mouth and arrange beds in tiers in the spawn running room.
    7. After 15-20 days, cut and remove the polythene bag and transfer the beds to cropping room.


  • Mushroom pin heads appear on 3rd day of opening of beds and mature in 3 days.
  • Harvest matured mushrooms daily or alternate days, before spraying water.
  • Second and third harvest can be obtained after scraping the surface of beds after first or second harvest.

Source: TNAU Agritech Portal

Related resources

Oyster video

A video on Mushroom Production Technology, Oyester, harvesting, infestation and building.
A video on Mushroom Production Technology, Oyster, harvesting, infestation and building.
M.POONKOTHAI Jul 15, 2019 04:00 PM

nice job

IVR.Ravi Jan 09, 2019 09:33 AM

Good morning. How you will control Rh and contamination in thatched hut.

reynaldo Sep 16, 2018 01:10 PM

Its suitable in tropical country like philippines im a begginer but i like to know better in producing mushrooms

Sanjay Ramachadran Apr 26, 2016 02:26 PM

Great Job.....Thank You

MOHAMMEDUL HAQUE May 06, 2014 12:40 PM

Add mushroom in your daily diet and live a healthy life free from high blood pressure,blood sugar,anaemia etc

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